If you're using tripod, after you have focused and composed and ready to release the shutter, lock the mirror first and wait for the camera to stop vibrating before releasing the shutter. If you don't see vibration, you probably don't need mirror lockup.
I only experience mirror vibration in 2 instances,
1) Astrophotography.
2) For handheld shots with VR lens at shutter speed around 1/4s to 2s. The hand will try to counter the mirror slap and correct, at the same time, the VR will try to counter the that movement also and blurs the image instead. I think it might be due to the way I shoot, so sometimes, I get a better picture with the VR off at that kind of shutter speed.
Otherwise, mirror vibrations doesn't bother me at all.