Roma captain Francesco Totti will miss Wednesday's Champions League return at Old Trafford after being omitted from the travelling squad.
The 31-year-old striker sat out Saturday's 3-2 home win over Genoa in Serie A. Roma coach Luciano Spalletti admitted then: "I would be happy to have him back next weekend."
Totti was conspicuous by his absence last Tuesday when United took a big stride towards the semi-finals, winning 2-0 with goals from Cristiano Ronaldo and Wayne Rooney.
Another first-leg absentee, Greater Manchester-born midfielder Simone Perotta, is recalled to the Roma ranks after completing a one-match suspension.
Spalletti named the following squad for the second leg, as listed on AS Roma's website:
Goalkeepers: Gianluca Curci, Doni, Carlo Zotti
Defenders: Christian Panucci, Marco Cassetti, Cicinho, Juan, Philippe Mexes
Midfielders: Gabriel Antunes, Alberto Aquilani, Matteo Brighi, Daniele De Rossi, Simone Perrotta, David Pizarro, Rodrigo Taddei, Max Tonetto
Forwards: Mauro Esposito, Ludovic Giuly, Alessandro Mancini, Mirko Vucinic.